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Association-dissociation curves of (a) unlabeled and (b) Cy3-labeled monovalent Fab fragment of goat IgG against the (H+L) domain of rabbit IgG with surface-immobilized rabbit IgG at different goat IgG concentrations. The curves are globally fitted (dot lines) to a two-site Langmuir reaction model (see main text for details) with the global fitting parameters listed in Table 3.
