Table 1.

Equilibrium dissociation constant K_D= k_off/k_on of unlabeled and Cy3-labeled streptavidin with linear peptide SAWSHPQFEK-linker-BSA conjugates. The conjugates are immobilized on epoxy-functionalized glass surface through primary amine groups on the BSA surface.

	θ(1)	${\mathrm{k}}_{\text{on}}^{(1)}$(M−1s−1)	${\mathrm{k}}_{\text{off}}^{(1)}$ (s−1)	$K_D^{(1)}$ (nM)	θ(2)	${\mathrm{k}}_{\text{on}}^{(2)}$ (M−1s−1)	${\mathrm{k}}_{\text{off}}^{(2)}$ (s)−1	$K_D^{(2)}$ (nM)
Unlabeled Streptavidin	0.71	1.7×103	1.6×10−4	92±10	0.29	3.3×104	6.4×10−3	192±8
Cy3-labeled Streptavidin	0.81	1.5×103	3.8×10−4	259±12	0.19	8.3×103	6.1×10−3	720±74