Table 4.

Equilibrium dissociation constant K_D = k_off/k_on of reaction of unlabeled and Cy3-labeled goat whole IgG (against the F_c domain of rabbit IgG) with surface-immobilized rabbit IgG targets.

	θ(1)	${\mathrm{k}}_{\text{on}}^{(1)}$ (M−1s−1)	${\mathrm{k}}_{\text{off}}^{(1)}$ (s−1)	${\mathrm{K}}_{\mathrm{D}}^{(1)}$ (nM)	θ(2)	${\mathrm{k}}_{\text{on}}^{(2)}$ (M−1s−1)	${\mathrm{k}}_{\text{off}}^{(2)}$ (s)−1	$K_D^{(2)}$ (nM)
Unlabeled goat IgG	0.33	3.6×104	2.5×10−5	0.70±0.13	0.67	2.4×103	3.2×10−5	13.6±1.9
Cy3-labeled goat IgG	0.22	4.1×104	1.3×10−4	3.20±0.44	0.78	2.5×103	6.9×10−6	2.81±0.22