Table 1.
Phenotypic changes of donor-derived human cells in HRC liver during PHx-induced liver regeneration
| Animal No. (age) |
Cell markers |
||||||||||
|
AFP |
CK19 |
CK18 |
CD34 |
CD45 |
|||||||
| I | II | I | II | I | II | I | II | I | II | ||
| 1 and 2 (2 mo) | A | - | - | + | - | - | + | - | - | - | - |
| B | - | + | + | - | - | + | - | + | - | - | |
| C | - | - | + | - | - | + | - | - | - | - | |
| 3 and 4 (2 mo) | A | - | - | + | + | + | - | + | - | - | - |
| B | - | - | + | + | + | - | + | + | - | + | |
| C | - | - | + | + | + | + | - | - | - | + | |
| 5 and 6 (2 mo) | A | - | - | + | + | + | - | - | - | - | - |
| B | - | + | + | + | + | + | - | + | - | + | |
| C | - | - | + | + | + | + | - | - | - | - | |
| 7 and 8 (2 mo) | A | - | - | - | + | + | + | - | - | - | - |
| B | - | - | - | + | + | + | - | - | - | - | |
| C | - | - | - | + | + | + | - | - | - | - | |
| 9 and 10 (2 mo) | A | - | - | - | - | + | + | - | - | - | - |
| B | - | - | - | - | + | + | - | - | - | + | |
| C | - | - | - | + | + | + | - | - | - | - | |
Animals 1-10 at the age of 2 mo with the engraftment of donor-derived hHLCs in the liver of HRC were screened by human gene-specific PCR for hAlu as previously described[19,20] and ISH for human Alu sequences confirmed by detecting human β2-M expression using IHC. Animals 2, 4, 6, 8 and 10 at the age of 2 mo underwent 70% PHx. Age-matched animals 1, 3, 5, 7 and 9 (not undergone PHx), were used as negative controls. In the human-rat chimeric liver, donor-derived human cells with different cellular phenotypes were detected by IHC for different markers (AFP, CD34, CD45, CK18 and CK19). Minimal liver tissues from PHx (II) and non-PHx (I) groups used for IHC were dissected and collected before PHx (A), and after 10 d (B) and 20 d (C) of PHx.