Figure 3.

Astragalus mongholicus polysaccharide inhibits p38 phosphorylation but not ERK1/2 or JNK activation in LPS-stimulated rat small intestinal cells. IEC were treated as described in Figure 1. After incubation in a medium containing 10 μg/mL LPS with APS at different concentrations of for 1 h, Western blotting analysis was performed to detect phosphorylated p38 (A), total p38 (B), phosphorylated ERK (C), total ERK (D), phosphorylated JNK (E), total JNK (F) and actin (G).