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. Author manuscript; available in PMC: 2009 Aug 5.
Published in final edited form as: Science. 2008 Aug 22;321(5892):1095–1100. doi: 10.1126/science.1155998

Fig. 3. Suppression of MyoD-induced muscle differentiation by HES1.

Fig. 3

Primary human fibroblasts (CCL153) were sequentially transduced with a MyoD-estrogen receptor fusion protein (MyoD-ER) and then with an empty vector (vector), wtHes1, or dnHes1. Proliferating cells were induced to differentiate by β-estradiol (SP), or were made quiescent for 4 days by serum starvation (SF) before induction of differentiation. (A) At 0 and 72hr after addition of β-estradiol, myosin heavy chain (MHC) RNA was measured by real-time PCR normalized to GAPDH levels. The fold change relative to differentiated vector cells is plotted. Data are the average of duplicates. RNA amounts below the detection limit are indicated as “0”. (B) The induction of MHC at 72 hrs post-differentiation is shown by immunoblot; and (C) by immunostaining using an anti-MHC antibody. MHC is green; nuclei are blue (DAPI). Scale bars represent 20 µm.