Table 1.

Cytokines in cultures from mice immunized with transgenes carrying individual Th cell determinants

Exp.*	DNA	Peptide in culture
		VTSA			NVDP
		IL-2	IFN-γ	IL-4	IL-2	IFN-γ	IL-4
1	γ1NV2VTSA3	1612  ± 472	290  ± 52	290  ± 118	1825  ± 654	334  ± 68	368  ± 126
	γ1VTSA3	<70	<50	<40	<70	<50	<40
	γ1NV2	<70	<50	<40	1950  ± 430	233  ± 30	186  ± 83
	γ1NV2/γ1VTSA3	862  ± 225	155  ± 53	<40	1537  ± 166	209  ± 56	<40
2	γ1NV2VTSA3	1182  ± 290	243  ± 48	235  ± 35	848  ± 219	243  ± 38	105  ± 26
	γ1NV2	<70	<50	<40	1171  ± 508	815  ± 444	58  ± 16
	γ1NV2 + γ1VTSA3	<70	<50	<40	450  ± 125	120  ± 7	<40

Spleen cells were harvested on day 14 after DNA immunization. Cells were cultured for 3 days in the presence of synthetic peptide. Supernatants were tested for cytokine content as detailed in Materials and Methods. Cytokines are expressed in pg/ml. Symbols: /, coinjected (mixing experiment); +, separately injected. 

^*Experiment 1 corresponds to experiment represented in Fig. 3a, and experiment 2 corresponds to experiment represented in Fig. 3b.