Table 2.

Temporal dynamics of cytokine production

Day	μg/ml	Peptide in culture
		VTSA			NVDP
		IL-2	IFN-γ	IL-4	IL-2	IFN-γ	IL-4
3	50	<70	<50	<40	<70	<50	<40
	16.5	<70	<50	<40	<70	<50	<40
	5.5	<70	<50	<40	<70	<50	<40
	1.8	<70	<50	<40	<70	<50	<40
5	50	<70	<50	<40	298  ± 65	1835  ± 224	80  ± 67
	16.5	<70	<50	<40	160  ± 55	2170  ± 340	116  ± 67
	5.5	<70	<50	<40	125  ± 32	568  ± 1076	<40
	1.8	<70	<50	<40	54  ± 13	225  ± 391	<40
7	50	1197  ± 689	466  ± 137	<40	1112  ± 484	1438  ± 247	<40
	16.5	455  ± 204	159  ± 119	<40	1082  ± 383	607  ± 256	<40
	5.5	349  ± 236	<50	<40	535  ± 168	84  ± 84	<40
	1.8	57  ± 12	<50	<40	250  ± 74	<50	<40

Mice were immunized with plasmid γ1NV²VTSA³. Spleen cells were harvested on day 14 after DNA immunization. Cells were cultured for 3 days in the presence of synthetic peptide. Supernatants were tested for cytokine content as detailed in Materials and Methods. Values correspond to experiment shown in Fig. 4. Cytokine levels are expressed in pg/ml.