Figure 1. Growth and lethality defects in itpr mutant larvae can be rescued by UASitpr⁺ expression in IPCs.

(A) Third instar larvae at 120 hrs After Egg Laying (AEL). UASitpr⁺/+;itpr^sv35/ug3 are significantly reduced in size and fat body content. Both Dilp2GAL4 and DdcGAL4 rescued larvae start wandering at this stage and appear similar to wild-type (Canton-S) controls. (B) Wild-type (Canton-S) and animals of all rescue conditions grown at 25°C survive better as compared to itpr mutants (UASitpr⁺/+;itpr^sv35/ug3) grown under the same conditions at later times after egg laying (*p<0.05; Student's t-test). For each time interval and genotype, 75 animals were screened in 3 batches of 25. Each bar represents the total viability of the indicated genotype. The colored subdivisions in each bar represent the number of larvae developing to later larval, pupal (P) or adult (A) stages. The survival profile of DdcGAL4 rescued animals (UASitpr⁺/+; DdcGAL4/+; itpr^sv35/ug3) is better than that of Dilp2GAL4 rescued condition (UASitpr⁺/+; Dilp2GAL4/+; itpr^sv35/ug3). Expression of UASitpr⁺ simultaneously with Dilp2GAL4 and DdcGAL4 (UASitpr⁺/+; Dilp2GAL4/DdcGAL4; itpr^sv35/ug3) does not improve survival beyond that observed with only DdcGAL4. Results are expressed as mean±SEM.