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. 2009 Aug 14;4(8):e6652. doi: 10.1371/journal.pone.0006652

Figure 5. Overlap of DdcGAL4 and Ddc labeled cells in larval brains.

Figure 5

Three-dimensional projections of confocal Z-stacks of a wild-type Drosophila 3rd instar larval brain (A–C) expressing mCD8GFP with DdcGAL4 and immunostained with an anti-Ddc antibody (A) and anti-GFP antibody (B). A majority of DdcGAL4::UASmCD8GFP labeled cells overlap with those stained with the Ddc antibody, though there are some cells in both cases that do not overlap (green arrows in B). These do not appear in the region of the IPCs. Green arrowhead in (B) indicates DdcGAL4::UASmCD8GFP expression in Ddc stained cells in the sub-esophageal ganglia that lie in close proximity to IPC projections. Scale bars A–C, 50 µm.