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. 2009 Aug 21;5(8):e1000614. doi: 10.1371/journal.pgen.1000614

Figure 5. Ablation of the Rap1 silencing domain increases the proportion of active RNA pol II on RP genes.

Figure 5

(A) RNA pol II levels, as detected with the 8WG16 antibody, on the RP genes are not significantly affected by the rap1Δsil mutation. (B) The rap1Δsil mutation increases the levels of active RNA pol II, as detected by run-on, on RP genes. (C) The proportion of active vs. total RNA pol II on the RP genes is significantly lower in the rap1-Δsil mutant than in the wild type (RAP1). (D) Although there is no significant difference in the distribution of total RNA pol II molecules within the RPS3 gene, there is a significant increase (E) of the active RNA pol II molecules toward the 3′ end of this gene in the rap1-Δsil mutant in relation to the wild type. Run-on data were normalized according to the signal of the probes of PRI2 present on the same filters, as described in Materials and Methods. The data shown in the histograms correspond to the average of two experiments. Error bars represent standard deviation. Text inserts are as in Figure 1.