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Primer sequence, length and annealing temperature of exons 5-8 in PTEN gene
| Primer sequence | Primer length (bp) | Amplification fragment length (bp) | Annealing temperature (°C) | |
| Exon-5F: | ACCTGTTAAGTTTGTATGCAAC | 22 | 379 | 52 |
| R | TCCAGGAAGAGGAAAGGAAA | 20 | ||
| Exon-6F: | CATAGCAATTTAGTGAAATAACT | 23 | 274 | 52 |
| R | GATATGGTTAAGAAAACTGTTC | 22 | ||
| Exon-7F: | TGACAGTTTGACAGTTAAAGG | 21 | 263 | 58 |
| R | GGATATTTCTCCCAATGAAAG | 21 | ||
| Exon-8F: | CTCAGATTGCCTTATAATAGTC | 22 | 558 | 52 |
| R | TCTGTTACTTGCTACGTAAAC | 21 |
