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. 2008 Jun 28;14(24):3819–3828. doi: 10.3748/wjg.14.3819

Figure 1.

Figure 1

A: Characterisation of the p53 status and analysis of parvoviral proteins in different human tumor cells by Western blot. Cells were cultured for 2 d and lysed with RIPA buffer, and 50 µg of total protein was subjected to SDS-PAGE. For p53 protein detection, blots were incubated with the monoclonal DO-7 antibody; B: Production of parvoviral proteins in H-1 PV-infected p53 different tumor cells. Hep3B4P and HepG2 cells were H-1 PV-infected (MOI = 20 pfu/cell) and grown for 1 to 3 d. After lysis with RIPA buffer, 50 μg of total proteins were equally diluted and separated on SDS-PAGE. For parvoviral protein detection, blots were incubated with the NS1-specific antibody[37].