Fig. 2.

Four gel pairs with individual grayscale images of the sham Cy3 dye and CCI Cy5 dye stained gels and a corresponding composite Sypro Ruby-stained gel of each paired sample. The Sypro Ruby-stained gel serves as a reference sample of both samples together to allow for the visualization of the spatial correlation between each paired sample and the image of the total proteins combined from each sample when imaged in the same gel. Gel quality used for the study and relative visual differences can be seen. Each column represents a single DIGE gel with a sham image (top row), injury image (middle row) and composite image (bottom row).