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PPARδ (A) mRNA and (B) protein expression in a panel of colon cancer cell lines. (A) Total RNA (20 μg) was isolated from eight different indicated colon carcinoma cell lines and analyzed for PPARδ mRNA expression by Northern blot hybridization. (B) Whole-cell protein lysates from the indicated cell lines (50 μg) were analyzed for PPARδ protein expression by using immunoblot analysis.
