Figure 5.

Transactivation of endogenous PPARδ in human colon cancer cells. (A) HCA-7 cells were transiently transfected with PPRE3-tk-luciferase and pRL-TK plasmids followed by treatment with increasing doses of the following ligands (published PPAR isoform selectivity in parenthesis): cPGI (PPAR α/δ), GW 2433 (PPAR α/δ), or GW 7647 (PPAR α) for 24 h. Cells were harvested and the dual luciferase assay was performed as described in Materials and Methods. Data are presented as -fold activation relative to vehicle treated (0.1% DMSO) and represent the mean from three independent transfections. Error bars equal SEM.