Figure 2.

SUMO-1-modified TEL localizes to the TEL bodies. 293 cells grown on coverslips were transiently transfected with HA-TEL and Flag-SUMO-1. The cells were fixed and immunostained with anti-HA rat/anti-rat-FITC antibodies and with anti-Flag mouse/anti-mouse Texas Red antibodies for HA-TEL detection (A) or for Flag-SUMO-1 detection (B). The images were overlapped to determine colocalization (C). The transfected 293 cells were treated as described and immunostained with anti HA antibody for TEL detection (D) and with anti-PML antibody for PML detection (E). (F) Overlap of D and E.