Figure 4.

The HLH domain of TEL is required for nuclear localization and SUMO-1 conjugation. (A) COS7 cells were transfected with HA-TELΔHLH alone (lane 1) or with HA-TELΔHLH and Flag-SUMO-1 (lane 2). After protein separation, the cell lysates were analyzed with anti-HA antibody. Absence of a SUMO-1-modified band in lane 2 indicates that the HLH domain of TEL is required for modification. (B) 293 cells transfected with HA-TEL (A) or with HA-TELΔHLH (B) were immunostained with anti-HA rat/anti-rat-FITC antibodies. TELΔHLH is present in the cytoplasm and nucleus of cells.