Figure 2. LowGM-DC remain functionally immature after maturation.

DC were generated under low-GM (A) and standard highGM plus IL-4 conditions (B) for 6 days, stimulated with the indicated reagents or a “cocktail” consisting of TNF, IL-1β, IL-6 and PGE₂ for 24 h and then added at titrated numbers to allogeneic T cells, as represented by non-adherent fraction of PBMC of an allogeneic donor. After 3 days [³H]-thymidine was added overnight to measure proliferation. The data from one experiment shown are representative of 3 independent experiments. Values represent the mean±standard deviation error bars of triplicate cultures from one experiment.