Figure 3. A single allogeneic T cell priming by lowGM-DC does not induce T cell anergy.

Allogeneic T cells were stimulated with either immature lowGM-DC or immature highGM+IL-4-DC or LPS-matured highGM+IL-4-DC. After 5 days the T cells were restimulated with IL-2 or the indicated antibodies or LPS-matured DC from the original allotype (matDC allo) or a third party allotype (matDC 3rd) or cells were left without stimulation (control) for another 3 days before [³H]-thymidine ([³H]-Th.) was added overnight to measure proliferation as shown in the flow chart. The data from one experiment shown are representative of 5 independent experiments. Values represent the mean±standard deviation error bars of triplicate cultures from one experiment.