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Evans Blue and TTC staining. Hearts were stained by Evans Blue and TTC to confirm the infarct area, the AAR and the ANAR after 30 min of ischaemia and 7 days of reperfusion. EP4RAG (3 mg/kg) treatment (B) significantly decreased infarct/AAR and infarct/LV compared with the vehicle-treated group (A). Statistical analyses of infarct size and AAR in hearts from the vehicle- and EP4RAG-treated rats (C). *P < 0.05 compared with rat given vehicle.
