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Total GSH quantification at 72 hpf, with and without 100μM NAC and 1mM BSO. NAC and BSO treatments spanned 24–72 hpf and PAH and vehicle control treatments spanned 48–72 hpf. A three-factor ANOVA for pretreatment, BNF, and ANF showed a significant effect of NAC (p = 0.03) and BSO (p < 0.0001). *indicates a significant difference (p ≤ 0.05) of either NAC or BSO pretreatment on GSH levels compared to no pretreatment controls within an exposure group; mean + SEM; n = 3 pools of 25 embryos.
