FIG. 4.

Treatment of mice with BSO (at 400 mg/kg/day for 16 days in the drinking water) substantially depletes GSH. Mice were treated with BSO or with normal water and then treated as noted in the legend for Figure 3 with SMD and LPS. Peritoneal macrophages were harvested 1 h after LPS administration, and GSH and GSSG in these cells were quantified as described in Materials and Methods section. Values shown are means ± SEM (group size = 5), and values significantly different from those for mice treated with LPS only are shown by * (p < 0.05), ** (p < 0.01), or *** (p < 0.001), as determined by ANOVA followed by the SNK post hoc test.