FIG. 2.

Kinetics and concentration-dependence of DON-induced GRP78 degradation in peritoneal macrophages. Following exposure to DON, peritoneal macrophages were cultured with DON (500 ng/ml) or water vehicle (VH) for various intervals. Total protein was extracted and GRP78 was analyzed by Western blot. (A) Kinetics of GRP78 degradation during exposure to 500 ng/ml DON. (B) Concentration-dependent DON-induced GRP78 degradation. RI indicates relative intensity which is the percentage of the maximal fluorescence in the same lane.