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Molecular events in isotype switching. CD40+ B cells were stimulated with anti-CD40+IL-4 using two concentrations of anti-CD40 (0.1 and 1 μg ml−1) and examined for (A) expression of Cε GLT, AID and Iμ-Cε mature transcripts by RT-PCR at day 4. (B) Semi-quantitative analysis of three experiments performed. Values represent the ratio of each transcript/β2 m.
