. 2009 Feb 13;63(4):675–678. doi: 10.1093/jac/dkp015

Table 1.

EhADH2 enzymatic activities are rescued by adding iron

Metal/chelator additives	ADH (U/mg)	ALDH (U/mg)
M9 medium only	1.31 ± 0.52	0.41 ± 0.19
30 µM Fe²⁺	27.88 ± 2.40	7.67 ± 1.19
50 µM Zn²⁺	0.23 ± 0.17	−0.03 ± 0.3
30 µM 1,10-phenanthroline	0.71 ± 0.46	−0.15 ± 0.8
30 µM 1,10-phenanthroline+30 µM Fe²⁺	28.83 ± 1.14	9.21 ± 1.24
30 µM 1,10-phenanthroline+30 µM Fe²⁺+50 µM Zn²⁺	22.67 ± 2.51	8.56 ± 0.39

NAD-dependent ADH and ALDH activities were measured from partially purified bacterial lysates of E. coli SHH31 expressing wild-type recombinant EhADH2 enzyme to which indicated metals, chelator or combined additives were added.