Fig. 2.

Both elevated FC and decreased CE are required for altered proteolytic processing of APP in AC29 cells. AC29 cells stably transfected with APP were treated ad equilibrium with methyl β-cyclodextrin (mβ-CD; 1 mM) and/or mevastatin (5 µM) to reduce FC levels. a Only the combination of mβ-CD and mevastatin reduced FC levels to those found in wild-type CHO cells. mβ-CD alone was able to partially reduce but did not normalize FC levels. b Normalization of FC increased but did not normalize the secretion of Aβ into the media, reaching ~50% of total Aβ secreted by wild-type CHO cells. c Western blot showing that both the ~55- and ~85-kDa APP-CTFs (shown by arrowheads) disappeared after normalization of FC levels (mβ-CD + mevastatin). This was accompanied by an increase in the steady-state levels of both C99 and C83. Antibody: C7, against the C-terminus of APP. Values are the mean±SD of at least three separate experiments. asterisks, p<0.05