Fig. 1.

C. neoformans phenotype affects biofilm formation. (a) Cells from smooth and mucoid phenotypes from four C. neoformans isolates were allowed to adhere to polystyrene plates and the amount of biofilm formed after 48 h was determined by using XTT assay. Bars are the averages of three measurements, and error bars denote standard deviations. Asterisks denote P value significance calculated by t-test. P values of <0.05 were considered significant. (b) Examples of colony phenotypes smooth and mucoid switch phenotypes originating from C. neoformans RC-2. F4-WR from 24067a and wrinkled and smooth from serotype A strain SB4. The colony morphologies were assessed after biofilms were disrupted and yeast cells were cultured on Sabouraud agar, and incubated for 48 h at 30°C