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. 2000 Nov 7;97(24):13294–13299. doi: 10.1073/pnas.230453097

Figure 1.

Figure 1

Ank/Aγ-globin retrovirus constructs. (A) The integrated single-copy provirus. The LTR sequences are shown as hatched boxes, the ankyrin promoter as a gray box, exon sequences in the γ-globin gene as dark boxes, and intron sequences as open boxes. The asterisk indicates the presence of a 717-bp deletion in IVS2 of the γ-globin gene. (B) The double-copy retrovirus vector in the producer cell genome (Upper) and the integrated provirus (Lower). The elements are identified as in A. Note that during the conversion of the RNA genome to DNA, the 3′ LTR, which includes the Ank/Aγ-globin gene, is duplicated into the 5′ LTR. The positions of the restriction enzyme sites for HindIII and EcoRV are shown. HindIII digestion detects intact 5′ LTR sequences and generates a specific 3′ junction fragment for each insertion. EcoRV digestion detects intact 3′ LTR sequences and a specific 5′ junction fragment for each insertion.