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RNase protection of Ank/Aγ-globin mRNA from CFU-S colonies containing the single- or double-copy Ank/Aγ-globin vector. One microgram of total RNA was analyzed in each lane except the K562 positive control lane, which contained 10 μg. (Upper) Bands protected by the Ank/Aγ-globin probe are shown (exon 1, 139 bp; exon 2, 175 bp). (Lower) The band protected by the mouse α-globin probe is shown (exon 2, 186 bp).
