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Southern blot analysis of single-copy (A) and double-copy (B) Ank/Aγ-globin retrovirus vectors in long-term repopulated mice. Each lane contains 10 μg of DNA isolated from the peripheral blood of mice. (A) HindIII digest. (B) EcoRV digest. The sizes of the bands indicated at the right indicate the predicted size of the intact provirus. +C: EcoRI-digested DNA from a transgenic mouse with one intact copy of the Ank/Aγ-globin gene.
