Figure 5.

RNase protection of Ank/^Aγ-globin mRNA from reticulocytes of long-term repopulated mice transplanted with bone marrow cells exposed to the single- or double-copy Ank/^Aγ-globin vectors. The transgenic mouse controls at the left have 1, 4, and 12 copies of the Ank/^Aγ-globin transgene and express Ank/^Aγ-globin mRNA at levels of approximately 3.7, 15, and 45% of mouse α-globin. (Upper) Bands protected by the Ank/^Aγ-globin probe are shown (exon 1, 139 bp; exon 2, 175 bp). (Lower) The band protected by the mouse α-globin probe is shown (exon 2, 186 bp).