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. 2009 Aug 10;4(8):e6562. doi: 10.1371/journal.pone.0006562

Figure 3. Recruitment of a distinct macrophage population in metastasis bearing lungs.

Figure 3

(A–D) Pulmonary metastasis of breast cancer cells are highly infiltrated with macrophages. Representative Mac3 immunohistochemistry staining of transverse sections of lung metastatic lesions from different tumor models: (A) experimental metastasis of primary PyMT tumor cells; (B) spontaneous metastasis derived from a MMTV-PyMT induced mammary tumor; (C) experimental metastasis of Met-1 cells and (D) spontaneous metastasis derived from subcutaneously implanted MDA-231 cells. Bar equals 20 um. (E) Representative flow diagram of CSF-1R-GFP positive cells from normal lung (upper panel) and metastasis bearing lung from experimental metastasis assay of Met-1 cells (lower panel). n = 5 (F) Representative flow diagram of CSF-1R-GFP positive cells from normal lung (upper panel) and lung bearing spontaneous metastasis from MMTV-PyMT induced mouse mammary tumor (lower panel). n = 3 (G) Recruitment of CD11b+Gr1- macrophages (F4/80+) in lungs with experimentally induced metastasis with Met-1 cells. Lungs were harvested at time indicated after tumor cell i.v. injection. Data are shown as mean+SEM. n = 3, *p<0.05 and **P<0.01. (H) Representative flow histograms of normal lung macrophages (F4/80+, blue dashed line) versus recruited macrophage population (F4/80+CD11b+Gr1-, red solid line) from lungs bearing Met-1 cell metastases stained with antibodies of different cell surface makers (indicated at the right side of the histogram). X axis indicates the fluorescent intensity, Y axis indicates the percentage of maximum cell number, MFI (top right panel) denotes representative mean fluorescent intensity (n = 3).