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The effect of multicopper oxidase and quinone inhibitors on in vitro Mn(II) oxidation.
| Inhibitor | Rate of Mn(II) oxidation in cell-free extracts of Erythrobacter sp. SD21 (% of activity without inhibition) |
|
|---|---|---|
| Cyanide | 100 µM, 1 mM | 76, 19 |
| o-phenanthroline | 10, 100 µM | 100, 68 |
| Azide | 100 µM, 1 mM | 33, 2 |
| Phenylhydrazine | 20 µM* | 0 |
in the absence of exogenously added 10 µM PQQ. In all other cases, Mn(II) oxidase activity is measured as described in Materials and Methods.
