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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: J Immunol. 2009 Mar 15;182(6):3866–3876. doi: 10.4049/jimmunol.0713949

Fig. 2. MMP-28 is expressed by Clara cells in the lung.

Fig. 2

(A) Immunofluorescence staining for epilysin in the airway epithelium from naïve WT mice (top panel) compared to MMP-28 mice (bottom panel). Nuclei were labeled with 4',6-diamidino-2-phenylindole (DAPI), demonstrating epilysin expression in the cytoplasm of airway epithelial cells. (B) Co-immunostaining of epilysin and CCSP (Clara cell marker). (C) MMP-28 mRNA from whole lung and LCM-retrieved bronchiolar epithelium. MMP-28 expression is enriched in LCM-retrieved bronchiolar epithelium compared to whole lung. Samples were normalized to β2-microglobulin. (D) Immunofluorescence staining for epilysin in the airway epithelium from naïve WT mice (0 h), and P. aeruginosa-treated mice harvested at 4 and 24 h after infection. Immunostaining demonstrates decreased epilysin levels in the airway epithelium from infected mice.