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De novo sequencing of CbAST-C2 by tandem mass spectrometry. (a) Nano-LC-ESI-Q-TOF MS/MS of the peptide SYWKQCAFNAVSCFamide (825.902+) from HPLC fraction of H. americanus brain extract. (b) LTQ FTICR MS/MS of putative CbAST-C2 from HPLC fraction of H. americanus brain extract and (c) synthetic peptide standard SYWKQCAFNAVSCFamide (825.902+). Almost identical fragmentation patterns are seen for (b) and (c), confirming the sequence assignment obtained in (a).
