Figure 7. Knocking–down the expression of cyclin A₂ in K562 cells significantly inhibited erythroid differentiation induced by low concentration DOX.

Cells were transfected with cyclin A₂ siRNA or not two hours prior to the addition of 0.4 µM DOX. Forty hours later, erythroid differentiation was scored by the benzidine staining method to determine the percentage of hemoglobin-positive K562 cells. Tests were done four times, counting a minimum of 300 total cells from at least three random microscope fields each. *p<0.05, **p<0.001 vs. control untreated cells by Student's t-test.