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. Author manuscript; available in PMC: 2010 Jul 30.
Published in final edited form as: J Neurosci Methods. 2009 May 15;181(2):170–177. doi: 10.1016/j.jneumeth.2009.05.002

Figure 5.

Figure 5

The requirement and optimal concentration of serum was tested in the culture. Serum was shown to be a necessary factor driving glial scar formation (p<.05 no serum condition vs. any condition with serum) but even very low amounts of serum could induce some scar formation. While there was a qualitative correlation between higher serum and greater scarring, there was no significant difference between conditions as long as some serum was present. Black bar indicates “Base Protocol” conditions.