Table 1.

Procedure		Original Protocol	Base Protocol
Seeding Media	Base	MEM	Neurobasal
	Serum	10% FBS,	-
		10% Horse Serum	-
	Supplements	1 g/L glucose,	-
		2 mM L-glutamine	2 mM L-glutamine
		1 mM sodium pyruvate	-
		100 μM nonessential amino acids	-
		50 U/mL penicillin	50 U/mL penicillin
		50 μg/mL streptomycin	50 μg/mL streptomycin
		-	B27
Plate Coating		Poly-D-Lysine	Poly-D-Lysine
		-	10ug/ml Fibronectin
Seeding Density		1 × 106 cells/ml in 0.5 ml/well	1 × 106 cells/ml in 1 ml/well
Cell Feeding		Add 0.5 ml media at day 3	-
		-	Add 10 ng/ml bFGF at day 3
Treatment Day		Day 7	Day 10
Treatment Media	Base	MEM	Neurobasal
	Serum	2% FBS,	10% FBS
		2% Horse Serum	-
	Supplements	2 mM L-glutamine	2 mM L-glutamine
		1 mM sodium pyruvate	-
		50 U/mL penicillin	50 U/mL penicillin
		50 μg/mL streptomycin	50 μg/mL streptomycin
		-	B27
Treatment Protocol		Change media and place microwire	Change media and place microwire
Culture stop day		Day 17 (10 days after treat)	Day 17 (7 days after treat)
Frequency of scar formation		<30%	~100%
Typical Scar Index		0–2	7–8