Table 2.

Procedure		Base Protocol	Control Protocol
Seeding Media	Base	Neurobasal	Neurobasal
	Serum	-	-
	Supplements	2 mM L-glutamine	2 mM L-glutamine
		50 U/mL penicillin	50 U/mL penicillin
		50 μg/mL streptomycin	50 μg/mL streptomycin
		B27	B27
Plate Coating		Poly-D-Lysine	Poly-D-Lysine
		10ug/ml Fibronectin	10ug/ml Fibronectin
Seeding Density		1 × 106 cells/ml in 1 ml/well	0.5 × 106 cells/ml in 1 ml/well
Cell Feeding		Add 10 ng/ml bFGF at day 3	Add 10 ng/ml bFGF at day 0
Treatment Day		Day 10	Any day 6–12 days after seed
Treatment Media	Base	Neurobasal	Neurobasal
	Serum	10% FBS	10% FBS
	Supplements	2 mM L-glutamine	2 mM L-glutamine
		50 U/mL penicillin	50 U/mL penicillin
		50 μg/mL streptomycin	50 μg/mL streptomycin
		B27	B27
Treatment Protocol		Change media and place microwire	Change media and place microwire. Add 10ng/ml bFGF.
Culture stop day		Day 17 (7 days after treat)	Any day 15–17 (at least 5 days after treat)