Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Aug 5;2009:834239. doi: 10.1155/2009/834239

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009 A. M. Eleuteri et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Effect of ELF-EMFs exposure and TPA treatment on protein oxidation. Protein oxidation was analysed and quantified by measuring the amount of protein carbonyl groups. The densitometric analysis from six separate blots provided for quantitative analysis of the amount of protein carbonyls is present (a) and a representative Western blot of protein carbonyls is shown (b). Equal protein loading was verified by using an antibody directed against GAPDH (c). Caco cells were cultured with and without 0.1 μM TPA and exposed to ELM-EMFs for 24, 48, and 72 hours. After harvesting and lysing the cells, samples were treated according to the OxyBlot procedure (Oxidized Protein Detection Kit, Oncor). Carbonyl groups were revealed by appropriate antibodies using an Enhanced Chemiluminescence Western blotting analysis system. Data points marked with an asterisk are statistically significant compared to their respective not exposed control cells (P < .05).