Skip to main content
. 2000 Nov 14;97(24):13312–13317. doi: 10.1073/pnas.230364197

Figure 1.

Figure 1

SMN modulates apoptosis of differentiated neural CSM 14.1.4 cells. (A) Map of the SV vector. (B) Cell viability of undifferentiated (31°C) and differentiated (39°C) CSM 14.1.4 cells was determined by propidium iodide/ Hoechst dual-staining after infection with the indicated recombinant SV. Data shown are for duplicate determinations in three independent experiments counting >400 cells per sample. Data from independently derived constructs were pooled. (C) Immunoblot analysis of undifferentiated CSM 14.1.4 cell lysates harvested at 24 h postinfection with the indicated recombinant viruses by using MANSMA2 anti-SMN antibody. Numbers in parenthesis distinguish two independently derived clones. (D) Individual clones of CSM 14.1.4 cells stably transfected with SMN, SMNΔ7, or green fluorescent protein were left undifferentiated (31°C) or were differentiated (39°C) and the number of colonies was determined 7 days after plating 100 cells. Results are the average of two similar independent experiments each using two independent clones per construct. (E) Cell viability was determined at 48 h postinfection of 293 and BHK cells, or at the indicated times after infection of primary rat cortical neurons with recombinant viruses. Data shown are the means ± SEM for duplicate determinations in three independent experiments counting >400 cells per sample.