Figure 6.

SMN-D252A has enhanced antiapoptotic function in vitro and in vivo. (A) Cell viability of undifferentiated (31°C) or differentiated (39°C) CSM 14.1.4 cells was determined as described for Fig. 1B at 48 and 72 h after infection with the indicated recombinant viruses. The means and SEM were determined in three independent experiments counting >300 cells per sample. (B) Representative fluorescence microscopy of differentiated CSM 14.1.4 cells stained with propidium iodide (red) and Hoechst (blue) at 72 h postinfection with recombinant viruses expressing SMN or derivatives. (C) Kaplan Meier survival analysis of 5-day-old mice infected with recombinant viruses as described in the legend to Fig. 2.