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. Author manuscript; available in PMC: 2009 Aug 6.
Published in final edited form as: J Immunol. 2007 Dec 15;179(12):8322–8331. doi: 10.4049/jimmunol.179.12.8322

FIGURE 5. Loss of Rap1a affects macrophage haptotaxis, adhesion and phagocytosis.

FIGURE 5

A. Adhesion of wild-type and Rap1a−/− macrophages to the (α4β1 integrin binding) fibronectin H296 peptide or vitronectin-coated cell culture wells was determined 90 min post plating following fixing and staining of adherent cells.

B. Haptotaxis of wild-type and Rap1a−/− macrophages through fibronectin H296 peptide-coated Transwell filters was measured at indicated time points.

C. Phagocytosis of IgG-coated sheep red blood cells by bone marrow-derived macrophages was measured at the indicated time points after exposure.

D. Phagocytosis of C3bi-opsonized sheep RBCs by macrophages was measured 30 min after exposure.

All data are mean +/− SD for cells derived from 3 mice/genotype and are representative of at least 4 independent experiments (2 experiments using a total of 4 mice were pooled for 5D).