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. Author manuscript; available in PMC: 2009 Aug 6.
Published in final edited form as: J Chromatogr A. 2006 Sep 1;1133(1-2):248–253. doi: 10.1016/j.chroma.2006.08.042

Fig. 2.

Fig. 2

Analytical RP-HPLC of crude sample mixture containing recombinant protein TM 1–99. Conditions: linear AB gradient (1% B/min) at a flow-rate of 0.3 ml/min, where Eluent A is 0.05% aq. TFA and Eluent B is 0.05% TFA in acetonitrile; temperature, 25 °C. P denotes desired product.