Skip to main content
. 2008 Oct 8;30(1):122–130. doi: 10.1093/carcin/bgn227

Fig. 6.

Fig. 6.

Senescence checkpoint genes are activated by chromatin remodeling agents 5-AzaC in esophageal keratinocytes. After 5-AzaC treatment, EPC-hTERT.D1-Cdx2 and MIGR1 (EPC.D1.X2 and EPC.D1.M, respectively) control cells exhibit characteristics of senescence including altered morphology, increased cell volume and subconfluent growth arrest in the normal medium. (A) Morphology of EPC-hTERT.D1.Cdx2 and control EPC-hTERT.D1.MIGR1 cells after 5AzaC at 1 and 5 μM for 5 days. Induction of senescence-associated β-galactosidase activity was identified by staining (blue). (B) 5-AzaC treatment induces the expression of senescence-associated p16(INK4a) and p21(Waf1/Cip1). Western Blot for p16 and p21 after cells were treated with 1 μM 5-AzaC or control diluent for 5 days. β-Actin served as an internal loading control. (C) Proposed model for the early molecular events preceding the onset of intestinalization in esophageal keratinocytes.