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. Author manuscript; available in PMC: 2010 Apr 25.
Published in final edited form as: Virology. 2009 Mar 9;387(1):157–167. doi: 10.1016/j.virol.2009.02.017

Figure 2. Northern blot confirmation of MR1 candidate.

Figure 2

(A) Northern blot analysis was conducted using the probes diagramed in red (left side of Fig.) on RNA harvested from mock or PyV-infected BMK cells at 50 h.p.i.. Arrows indicate bands corresponding to miRNAs. Note predominant band detected by all three probes migrating at ~65 nucleotides corresponds to the pre-miRNA. Bottom panel shows pre-transferred, low molecular weight RNAs stained with ethidium bromide as a loading control (B) Time course of pre-miRNA/miRNA accumulation (hybridized with 5′ probe).