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. 2009 Jul 29;422(Pt 1):23–35. doi: 10.1042/BJ20090428

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© 2009 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) The indicated GST-tagged fusion proteins (10 μM) were included with the primary anti-PtdIns4P antibody and anti-giantin antibodies applied to COS-7 cells. Staining conditions are as Figure 3. Single confocal optical sections are shown. Scale bar=20 μm. (b) Quantification of anti-PtdIns4P antibody labelling (n=207). See the Experimental section for details. ***P<0.001.