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. 2009 Jul 31;101(Pt 10):587–598. doi: 10.1042/BC20090021

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© 2009 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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12.5 and 14.5 dpc germ cell populations were assessed for gene expression of the germ cell marker Mvh, and the somatic cell markers Sox9 (XY) and Fst (XX). qPCR analysis from purified populations normalized to 18S RNA. n=3, error bars represent S.E.M. Highest expression of Mvh was detected in all germ cell populations. Expression of Sox9 was highest in the XY somatic cell populations, with greatest specificity at 14.5 dpc. Expression of Fst was also highest in the XX somatic cell populations, with greatest specificity at 14.5 dpc.