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. 2009 Aug 3;206(8):1787–1802. doi: 10.1084/jem.20090641

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© 2009 Pilichou et al.

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Figure 6. — Histological features of explanted hearts from Tg-NS/H mice. (A–O) Heidenhain trichrome, H&E, and von Kossa staining of transverse full sections of the hearts at different ages: <2 (A–C), 2.7 (D–F), 3.6 (G–I), 8 (J–L), and 12 (M–O) wk. Note the appearance of myocardial damage in the outer subepicardial layers with calcification in both RV and LV free walls after 2 wk of age, with progressive extension to become transmural by 4 wk of age, and deposition of mature fibrosis with wall thinning and aneurysm formation by 8 wk of age. Bars: (A–C) 0.5 mm; (D–F) 0.8 mm; (G–I) 0.5 mm; (J–L) 0.4 mm; and (M–O) 0.8 mm.